An observational study of brain blood vessel function in cerebral small vessel disease
| ISRCTN | ISRCTN10514229 |
|---|---|
| DOI | https://doi.org/10.1186/ISRCTN10514229 |
| Secondary identifying numbers | 1.0 |
- Submission date
- 06/05/2016
- Registration date
- 23/05/2016
- Last edited
- 09/06/2025
- Recruitment status
- No longer recruiting
- Overall study status
- Completed
- Condition category
- Circulatory System
Plain English summary of protocol
Background and study aims
Stroke and dementia are among the most pressing health issues in Europe, affecting a large proportion of the population. Cerebral small vessel disease (SVD) is a major cause these conditions, accounting for more than 30% of strokes and at least 40% of dementia cases. It is thought to happen because of damage to the lining of the tiny blood vessels in the brain that prevent them from working properly, causing damage to brain tissue. Progress in understanding the mechanisms behind SVD has been slow, and there are currently no proven effective treatments. Investigate@SVDs is part of a coordinated programme (SVDs@target – funded through the European Union’s Horizon 2020 research and innovation programme) designed to try and undertand the key mechanisms of different SVDs and how they contribute to individual SVDs. The aim of this project is to assess factors that drive brain microvascular dysfunction in different types of SVD.
Who can participate?
Adults who are able to have an MRI scan and have had a stroke due to SVD, have SVD associated cognitive impairment or have a genetic variant of SVD.
What does the study involve?
Participants visit the hospital at the start of the week to answer some questions about their health and have their blood pressure taken. A blood test is also taken and sent to a local laboratory to measure cells and chemicals in the blood that indicate if there is any inflammation. Then a blood pressure cuff is put around their arm which they will wear for a week while doing their usual activities, to continuously measure blood pressure. The recordings are sent anonymously via a mobile phone signal to a central computer for analysis. At the end of that week, participants attend another hospital visit to have a magnetic resonance brain scan to test the blood vessel leakiness and function. The scan lasts about two hours altogether, with a break in the middle. For the first part of the scan, the participant wears a face mask connected to some tubing. During the scan, for three minute intervals participants alternatively breath carbon dioxide mixed with air, and air alone (the carbon dioxide stimulates the brain blood vessels to open up and this can be detected on the scan). In the second part of the scan, after a break, participants have a small plastic tube placed into the vein at the elbow. After the next scan starts magnetic dye will be injected into the arm vein, which circulates to the brain with the blood and the amount that leaks out of the blood vessels is measured by the scanner. After this scan has finished, the stiffness of the main blood vessels in the neck and arm are measured while the patient is lying on a couch. This is performed using a small camera that sits on the skin over the blood vessel and takes a few minutes.
What are the possible benefits and risks of participating?
There is no direct benefit of taking part in the study. Risks of participating are low as all scanning methods used are well established and all participants will be screened carefully for things that mean they shouldn’t have the scans before they start. There is a small risks of pain and bruising during and after blood testing.
Where is the study run from?
1. Centre for Clinical Brain Sciences, Edinburgh (UK)
2. Ludwig-Maximilians University (Germany)
3. Maastricht University Medical Centre (Netherlands)
When is the study starting and how long is it expected to run for?
June 2017 to June 2019
Who is funding the study?
European Union Horizon 2020 (Belgium)
Who is the main contact?
Professor Joanna Wardlaw
joanna.wardlaw@ed.ac.uk
Contact information
Scientific
Centre for Clinical Brain Sciences
Chancellor’s Building
Little France Crescent
Edinburgh
H16 4SB
United Kingdom
 ORCID ID |
0000-0002-9812-6642 |
|---|---|
| Phone | +44 (0)131 332 2943 |
| joanna.wardlaw@ed.ac.uk |
Study information
| Study design | Cross sectional study |
|---|---|
| Primary study design | Observational |
| Secondary study design | Cross sectional study |
| Study setting(s) | Hospital |
| Study type | Diagnostic |
| Participant information sheet | Not available in web format, please use the contact details to request a patient information sheet. |
| Scientific title | Imaging NeuroVascular, Endothelial and STructural Integrity in prepAration to TrEat Small Vessel Diseases |
| Study acronym | INVESTIGATE@SVDs |
| Study objectives | 1. Greater blood brain barrier permeability will be associated with more reduced CVR 2. It will be possible to have increased BBB permeability without decreased CVR as this should occur at an earlier point in the pathogenesis of SVD 3. Increased enlarged perivascular spaces on structural imaging will correlate with reduced microvascular function 4. More variable blood pressure will worsen BBB permeability and CVR, and this effect will be greater than the effect of hypertension alone |
| Ethics approval(s) | South East Scotland Research Ethics Committee 01, ref: 16/SS/012 |
| Health condition(s) or problem(s) studied | Sporadic small vessel disease - stroke, cognitive impairment |
| Intervention | After answering some questions about their health, the subjects will have a blood pressure cuff put on their arm to wear for a week while doing their usual activities. This measures blood pressure continuously by day and night. At the end of that week, the subjects will come for a magnetic resonance brain scan to test the blood vessel leakiness and function. The scan lasts about two hours altogether with a break in the middle. The blood pressure measurement cuff is removed. During the scan, the subject will lie in the scanner. For the first part, they will wear a face mask connected to some tubing. Some images of the brain to show size and shape will be taken. Then the subject will breath carbon dioxide mixed with air through the mask for three minutes, then air, then the carbon dioxide+air, then air. The carbon dioxide stimulates the brain blood vessels to open up and this can be detected on the scan. After the first part of the scan, the mask will be removed and the subject can have a break. Then they go back in the scanner and a small plastic tube is put into the vein at the elbow, like for a blood test. After the next scan starts, some magnetic dye will be injected into the arm vein while the scan continues. The dye goes to the brain in the circulation and the amount that leaks out of the blood vessels over the next 20 minutes can be measured with the scanner. After this scan has finished, the subject will come out of the scanner and lie on a couch to have a measurement of the stiffness of the main blood vessels in the neck and arm. This is performed using a small camera that sits on the skin over the blood vessel. This takes a few minutes. Then the subject leaves and the study is finished. |
| Intervention type | Other |
| Primary outcome measure | Blood brain barrier leakiness is measured during the magnetic resonance scan from the gadolinium contrast agent uptake in the brain over 20 minutes after intravenous injection. The resulting contrast-time curve is modelled statistically to calculate contrast retention (leak) in different areas of the brain. |
| Secondary outcome measures | 1. Cerebrovascular reactivity to inhaled carbon dioxide is measured once prior to blood brain barrier leakiness 2. Blood pressure (systolic, diastolic, mean blood pressure, pulse pressure, and variability in blood pressure) is measured continuously for seven days prior to blood brain barrier leakiness scanning 3. Vascular stiffness is measured immediately after blood-brain barrier leakiness scanning using pulse wave velocity |
| Overall study start date | 30/06/2017 |
| Completion date | 30/05/2020 |
Eligibility
| Participant type(s) | Patient |
|---|---|
| Age group | Adult |
| Lower age limit | 18 Years |
| Sex | Both |
| Target number of participants | 75 |
| Total final enrolment | 78 |
| Key inclusion criteria | 1. Age 18 years or older 2. Able to undergo MRI 3. Capacity to give written informed consent 4. Symptomatic SVD defined as: 4.1. A history of clinical lacunar stroke syndrome in the last 5 years with a recent small subcortical infarct visible on MRI scan or CT scan* compatible with the clinical syndrome. OR 4.2. A diagnosis of CADASIL established by molecular genetic testing of the NOTCH3 gene (presence of an archetypical, cysteine-affecting mutation) or the presence of granular osmiophilic material (GOM) in ultrastructural, electron microscopy analysis of skin biopsy *On MRI, a recent infarct is defined as a DWI lesion on the acute MRI scan. On CT, recent infarct is defined as a novel lacune on CT within 3 weeks after the event that was not visible on the admission CT. or cognitive impairment defined as visiting a memory clinic and a clinical dementia rating [CDR] score of ≥ 0.5, and capacity to consent with confluent deep white matter hyperintensities (WMH) on MRI (defined on the Fazekas scale as deep WMH score ≥ 2) |
| Key exclusion criteria | 1. Inclusion criteria are not met 2. Unwillingness or inability to give written consent 3. Pregnant or breastfeeding women, women of childbearing age not taking contraception. 4. Acceptable contraception in women of childbearing age is a “highly effective” contraceptive measure as defined by the Clinical Trials Facilitation Group (http://www.hma.eu/fileadmin/dateien/Human_Medicines/01-About_HMA/Working_Groups/CTFG/2014_09_HMA_CTFG_Contraception.pdf) and includes combined (oestrogen and progesterone containing) or progesterone-only contraception associated with inhibition of ovulation, or intrauterine device or bilateral tubal occlusion 5. Contraindications to MRI (pacemaker, aneurysm clip, cochlear implant etc.) 6. Contraindications to gadolinium contrast agent used for MRI 7. Other major neurological or psychiatric conditions affecting the brain and interfering with the study design (e.g. multiple sclerosis) 8. In case of clinical lacunar stroke syndrome other causes of stroke such as: 8.1. ≥50% luminal stenosis (NASCET) in large arteries supplying the area of ischaemia 8.2. Major-risk cardioembolic source of embolism (permanent or paroxysmal atrial fibrillation, sustained atrial flutter, intracardiac thrombus, prosthetic cardiac valve, atrial myxoma or other cardiac tumours, mitral stenosis, recent (<4 weeks) myocardial infarction, left ventricular ejection fraction less than 30%, valvular vegetations, or infective endocarditis) 8.3. Other specific causes of stroke identified (e.g. arteritis, dissection, migraine/vasospasm, drug misuse) 9. Other stroke risk factor requiring immediate intervention that would preclude involvement in the study 10. Renal impairment (eGFR <30 ml/min) |
| Date of first enrolment | 30/04/2018 |
| Date of final enrolment | 31/07/2019 |
Locations
Countries of recruitment
- Germany
- Netherlands
- Scotland
- United Kingdom
Study participating centres
Little France Crescent
Edinburgh
EH16 4SB
United Kingdom
Feodor-Lynen-Strasse 17
Munich
81377
Germany
Maastricht University Medical Center
PO Box 5800
Maastricht
6202 AZ
Netherlands
Sponsor information
Hospital/treatment centre
University of Edinburgh & NHS Lothian
ACCORD
The Queen’s Medical Research Institute
47 Little France Crescent
Edinburgh
EH16 4JT
Scotland
United Kingdom
| Phone | +44 (0)131 242 6226 |
|---|---|
| susan.shepherd@nhslothian.scot.nhs.uk | |
| Website | www.accord.ed.ac.uk |
"ROR" |
https://ror.org/01x6s1m65 |
Funders
Funder type
Research organisation
Government organisation / National government
- Alternative name(s)
- EU Framework Programme for Research and Innovation, Horizon 2020 - Research and Innovation Framework Programme, European Union Framework Programme for Research and Innovation
Results and Publications
| Intention to publish date | 31/12/2021 |
|---|---|
| Individual participant data (IPD) Intention to share | Yes |
| IPD sharing plan summary | Available on request |
| Publication and dissemination plan | Current publication and dissemination plan as of 15/01/2020: Planned publication in peer-reviewed journals and presentations at national and international conferences. Early results will be presented at the European Stroke Conference 2020 Previous publication and dissemination plan: Planned publication in peer-reviewed journals and presentations at national and international conferences. |
| IPD sharing plan | The datasets generated during and/or analyzed during the current study are/will be available upon request from Joanna Wardlaw and the University of Edinburgh. The data will not be available until the primary analysis is complete and published. |
Study outputs
| Output type | Details | Date created | Date added | Peer reviewed? | Patient-facing? |
|---|---|---|---|---|---|
| Basic results | 25/06/2021 | 25/06/2021 | No | No | |
| Protocol article | 26/06/2021 | 16/08/2022 | Yes | No | |
| Abstract results | 01/09/2021 | 05/06/2024 | No | No | |
| Other publications | 09/12/2024 | 09/06/2025 | Yes | No |
Additional files
- ISRCTN10514229_BasicResults_25Jun2021.pdf
- Uploaded 25/06/2021
Editorial Notes
09/06/2025: Publication reference added.
05/06/2024: Abstract added.
18/10/2022: Internal review.
16/08/2022: Publication reference added.
25/06/2021: A basic results summary has been uploaded to the record.
21/06/2021: The intention to publish date has been changed from 31/12/2020 to 31/12/2021.
15/01/2020: The following changes have been made:
1. The recruitment end date has been changed from 31/12/2019 to 31/07/2019.
2. The total final enrolment number has been added.
3. The publication and dissemination plan was updated.
12/03/2019: Internal review.
19/02/2019: The following changes were made:
1. The recruitment end date was changed from 31/01/2019 to 31/12/2019
2. The overall end date was changed from 30/06/2019 to 30/05/2020
3. The intention to publish date was changed from 31/01/2020 to 31/12/2020
28/11/2018: The following changes were made to the trial record:
1. The overall trial start date was changed from 01/09/2016 to 30/06/2017.
2. The recruitment start date was changed from 05/07/2017 to 30/04/2018.
3. The recruitment end date was changed from 31/12/2018 to 31/01/2019.
4. The intention to publish date was changed from 30/06/2020 to 31/01/2020.
22/09/2017: Internal review.
12/09/2017: Recruitment dates have been updated from 01/10/2016- 30/09/2017 to 05/07/2017-31/12/2018. Overall trial end date has been updated from 30/12/2017 to 30/06/2019. Individual patient level data sharing statement has been added.
31/08/2017: Ethics information added.
