Minimizing nocebo effects by conditioning with verbal suggestion
| ISRCTN | ISRCTN76895197 |
|---|---|
| DOI | https://doi.org/10.1186/ISRCTN76895197 |
| Secondary identifying numbers | NL47084.058.14 |
- Submission date
- 16/10/2016
- Registration date
- 18/10/2016
- Last edited
- 08/11/2023
- Recruitment status
- No longer recruiting
- Overall study status
- Completed
- Condition category
- Signs and Symptoms
Plain English summary of protocol
Background and study aims
In common conditions associated with chronic (long-term) itching, the effects of treatment are usually small and vary between patients. Patients' expectations about treatment may contribute to this variation. Placebo effects are favorable treatment effects that are caused by patients’ positive expectations about a treatment. Nocebo effects are unfavorable treatment effects that are caused by patients’ negative expectations. Placebo and nocebo effects can be caused by verbal suggestion and conditioning. Verbal suggestion consists of providing verbal or written information about clinical improvement or aggravation, such as potential side effects. Conditioning consists of learning via experiencing. Placebo and nocebo effects have been investigated mainly in studies that focus on pain. Recent research has shown that verbal suggestion and conditioning can also induce placebo and nocebo effects on itching. This is highly relevant for the development of treatments for patients suffering from chronic itch, but first needs to be tested in healthy volunteers with itching stimulated either electrically or with the chemical histamine. The main aim of this study is to determine whether nocebo effects for electrical itch stimuli can be reduced by creating positive expectations, resulting in less itching. The other aims are to explore the effects of expectation on scratching behavior, and the role of characteristics like personality traits and genes.
Who can participate?
Healthy volunteers between 18 and 35 years old
What does the study involve?
The study consists of three parts. In part I, negative expectations regarding electrical itch stimuli are created. In the learning phase, two colored lights are displayed on a computer screen. Participants are instructed that one light indicates an increase in the itch stimulus and the other light indicates no change in the itch stimulus. Itch stimuli of high intensity are applied along with the first light and itch stimuli of medium intensity along with the other light. In the testing phase, participants are again told that the different lights indicate increased or non-changed itch stimuli, but only itch stimuli of medium intensity are applied.
In part II, participants are randomly allocated to one of three groups. In group 1 positive expectations are created by instructing participants that one light indicates a decrease in the itch stimulus and the other light indicates no change in the itch stimulus. Itch stimuli of low intensity are applied along with the first light and itch stimuli of medium intensity along with the other light. In group 2 negative expectations are created using the same procedure as Part I. In group 3 no verbal suggestion is provided and only itch stimuli of medium intensity are applied. In the testing phase only itch stimuli of medium intensity are applied for all three groups. The levels of itching and scratching behavior caused by the different stimuli are measured to find out whether the nocebo effects created during Part I of the study can be changed by creating positive expectations (group 1), resulting in lower itch scores.
In part III, the same verbal suggestions are given to each group as in part II and then a single histamine itch stimulus is given.
What are the possible benefits and risks of participating?
Participants are reimbursed with money or research credits. No risks are involved with participation in this study, only an investment of time. Sensations of itch are induced using frequently used and tested stimuli of short duration which are not burdensome.
Where is the study run from?
Leiden University (Netherlands)
When is the study starting and how long is it expected to run for?
September 2013 to July 2015
Who is funding the study?
Innovation Scheme (Vidi) Grant of the Netherlands Organization for Scientific Research
Who is the main contact?
Prof. Dr Andrea WM Evers
a.evers@fsw.leidenuniv.nl
Contact information
Scientific
Wassenaarseweg 52
Leiden
2333 AK
Netherlands
| Phone | +31 (0)71 527 6891 |
|---|---|
| a.evers@fsw.leidenuniv.nl |
Study information
| Study design | Single-centre three-arm randomized controlled single-blind study |
|---|---|
| Primary study design | Interventional |
| Secondary study design | Randomised controlled trial |
| Study setting(s) | Other |
| Study type | Treatment |
| Participant information sheet | Not available in web format, please use the contact details to request a patient information sheet. |
| Scientific title | Can nocebo effects on itch be modified by a positive expectation induction? A randomised controlled trial |
| Study objectives | The primary objective of the study is to determine whether induced negative expectation effects for electrical itch stimuli can be modified. It is hypothesized that levels of itch (measured on a numerical rating scale; NRS) will be reduced after positive expectation induction in comparison to control groups in which either continued negative expectations are induced or an extinction procedure is applied. Exploratively, also expectancy effects on scratching behavior and generalization of expectancy effects to another itch stimulus (histamine iontophoresis) are studied. It is explored whether scratching will be reduced after positive expectation induction in comparison to the control groups, and that levels of itch as well as scratching will also be reduced when histamine iontophoresis is applied. |
| Ethics approval(s) | Leiden University Medical Centre ethics committee (Commissie Medische Ethiek [CME]), 26/02/2014, ref: P14.019 |
| Health condition(s) or problem(s) studied | Experimentally induced itch in healthy subjects |
| Intervention | Participants visit Leiden University once for approximately 4.5 hours. Expectations regarding itch stimuli are induced by conditioning and verbal suggestion procedures with respect to purple and yellow colored cues being displayed on a computer screen. For half of the participants, the purple cue is the conditioned cue and the yellow cue is the neutral cue, and visa versa for the remaining participants. In part I during the learning phase, negative expectations for itch are induced in all participants by instructing them that the conditioned cue will indicate an increase in the itch stimulus. Accordingly, itch stimuli of high intensity are applied along with the conditioned cues and itch stimuli of medium intensity along with the neutral cues. In the testing phase, the same suggestions are provided, but only itch stimuli of medium intensity are applied. In part II, participants are allocated to one of three groups through stratified random allocation to ensure equivalent numbers of male and female participants in each group. The participants were blinded for allocation to these groups, but it was not possible to blind the experimenter since the experimenter provided different verbal suggestions for the different groups. 1. In group 1 (experimental), positive expectations are induced by conditioning and verbal suggestion. Participants are now instructed that the conditioned cue will indicate a decrease in the itch stimulus and accordingly itch stimuli of low intensity are applied along with the conditioned cues and itch stimuli of medium intensity along with the neutral cues. 2. In group 2 (control), the negative expectation induction is continued as in Part I 3. In group 3 (control), an extinction procedure is applied in which no verbal suggestion is provided and only itch stimuli of medium intensity are applied. In the testing phase of part II only itch stimuli of medium intensity are applied for all three groups. In part III, the same verbal suggestions are provided for each group as in part II and then a single histamine itch stimulus is provided. |
| Intervention type | Behavioural |
| Primary outcome measure | Level of itching, measured using an NRS score, induced by each electrical stimulus |
| Secondary outcome measures | 1. Scratching behavior, induced by each electrical stimulus. Scratching behaviour is assessed by videotaping the participants during the experiment. Frequency, duration and localization of scratching behaviour are scored by an experimenter who is blinded for the randomization of groups and colored cues. 2. Level of itching (NRS rating) and scratching behavior induced by histamine iontophoresis |
| Overall study start date | 04/09/2013 |
| Completion date | 16/07/2015 |
Eligibility
| Participant type(s) | Healthy volunteer |
|---|---|
| Age group | Adult |
| Lower age limit | 18 Years |
| Upper age limit | 35 Years |
| Sex | Both |
| Target number of participants | 99 |
| Total final enrolment | 129 |
| Key inclusion criteria | 1. Healthy human volunteers 2. 18 - 35 years old 3. Both male and female 4. Fluent in Dutch language |
| Key exclusion criteria | 1. Severe morbidity (e.g., multiple sclerosis, diabetes mellitus, heart or lung diseases) 2. Psychiatric disorders (e.g., depression) 3. Use of pacemaker 4. Color blindness 5. Diagnose of histamine hypersensitivity 6. Chronic itch or pain complaints 7. Pregnancy |
| Date of first enrolment | 10/09/2014 |
| Date of final enrolment | 16/07/2015 |
Locations
Countries of recruitment
- Netherlands
Study participating centre
Leiden
2333 AK
Netherlands
Sponsor information
University/education
Wassenaarseweg 52
Leiden
2333 AK
Netherlands
| Phone | +31 (0)71 527 6891 |
|---|---|
| a.evers@fsw.leidenuniv.nl | |
"ROR" |
https://ror.org/027bh9e22 |
Funders
Funder type
Government
Government organisation / National government
- Alternative name(s)
- Netherlands Organisation for Scientific Research, Dutch National Scientific Foundation, Dutch National Science Foundation, Dutch Research Council (Nederlandse Organisatie voor Wetenschappelijk Onderzoek), NWO:Nederlandse Organisatie voor Wetenschappelijk Onderzoek, Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NWO), Dutch Research Council, Dutch Research Council, Netherlands, NWO
- Location
- Netherlands
Results and Publications
| Intention to publish date | 01/12/2016 |
|---|---|
| Individual participant data (IPD) Intention to share | No |
| IPD sharing plan summary | Data sharing statement to be made available at a later date |
| Publication and dissemination plan | It is intended to publish the results in December 2016. |
| IPD sharing plan | The current data sharing plans for the current study are unknown and will be made available at a later date. |
Study outputs
| Output type | Details | Date created | Date added | Peer reviewed? | Patient-facing? |
|---|---|---|---|---|---|
| Results article | 14/09/2017 | 29/01/2019 | Yes | No | |
| Results article | 05/11/2018 | 29/01/2019 | Yes | No | |
| Dataset | 14/09/2017 | 08/11/2023 | No | No | |
| Protocol (other) | 14/09/2017 | 08/11/2023 | No | No |
Editorial Notes
08/11/2023: Protocol, dataset and total final enrolment added.
29/01/2019: Publication reference added
