Acute non-local effects of static stretching of the thigh muscles on drop jumping performance

ISRCTN ISRCTN12240843
DOI https://doi.org/10.1186/ISRCTN12240843
Sponsor University of Palermo
Funder Università degli Studi di Palermo
Submission date
14/11/2025
Registration date
31/03/2026
Last edited
31/03/2026
Recruitment status
No longer recruiting
Overall study status
Completed
Condition category
Musculoskeletal Diseases
Prospectively registered
Protocol
Statistical analysis plan
Results
Individual participant data
Record updated in last year

Plain English summary of protocol

Background and study aims
The study investigates how stretching the muscles in the legs affects flexibility and jumping performance. Researchers aim to understand the short-term impact of static stretching on these abilities in healthy young adults.

Who can participate?
Adults aged 18 or older who are physically active but not competitive athletes. Participants do not have muscle, joint, or nerve problems.

What does the study involve?
Participants attend one session in a laboratory. During this session, they complete flexibility and jumping tests before and after a stretching routine, and again 15 minutes later.

What are the possible benefits and risks of participating?
There is very little risk, although participants might feel mild discomfort while stretching. They may improve flexibility and help researchers learn more about sports science.

Where is the study run from?
The University of Palermo in Italy.

When is the study starting and how long is it expected to run for?
September 2022 to March 2023.

Who is funding the study?
The University of Palermo in Italy.

Who is the main contact?
Mr Antonino Scardina, University of Palermo – antonino.scardina01@unipa.it

Contact information

Mr Antonino Scardina
Principal investigator, Scientific

Sport and Exercise Sciences Research Unit
Department of Psychology, Educational Science and Human Movement
University of Palermo
Via Giovanni Pascoli 6
Palermo
90144
Italy

ORCiD logoORCID ID 0000-0001-9136-320X
Phone +39 91 2389 9605
Email antonino.scardina01@unipa.it
Prof Marianna Bellafiore
Public, Scientific

Sport and Exercise Sciences Research Unit
Department of Psychology, Educational Science and Human Movement
University of Palermo
Via Giovanni Pascoli 6
Palermo
90143
Italy

ORCiD logoORCID ID 0000-0002-3092-1666
Phone +39 91 2389 9605
Email marianna.bellafiore@unipa.it

Study information

Primary study designInterventional
AllocationRandomized controlled trial
MaskingOpen (masking not used)
ControlDose comparison
AssignmentCrossover
PurposeBasic science, Treatment
Scientific titleAcute non-local effects of static stretching of the thigh muscles on drop jumping performance
Study objectives To evaluate the effects of static stretching on flexibility and neuromuscular performance in healthy young adults.
Ethics approval(s)

Approved 25/11/2021, University of Palermo Bioethical Committee (Piazza Marina n. 61, Palermo, 90133, Italy; +39 91 238 93853; comitato.bioetica@unipa.it), ref: 65/2021

Health condition(s) or problem(s) studiedHealthy young adults without musculoskeletal or neurological disorders; study focuses on normal physiological responses to static stretching.
InterventionParticipants perform a static stretching protocol targeting the hamstring muscles. The protocol consists of repeated static stretches held for 30 seconds each, with controlled intensity below the discomfort threshold. The intervention is performed under supervision in a laboratory setting.
A control condition involving rest or no stretching is included to compare the physiological and functional responses.

Randomisation was performed using an online tool (www.randomizer.org). The platform generated a concealed allocation sequence assigning participants to the five conditions; group allocation was revealed only upon arrival at the laboratory. Due to the nature of the interventions, neither participants nor investigators were blinded.
Intervention typeBehavioural
Primary outcome measure(s)
  1. Hamstring flexibility measured using Passive straight leg raise (SLR) angle measured with a goniometer or motion analysis system to assess range of motion at baseline (T0), immediately post-intervention (T1), and 15 min post-intervention (T2)
  2. Jump performance measured using Peak jump height and flight time during single-leg drop jump measured using an Optojump optical measurement system. at baseline (T0), immediately post-intervention (T1), and 15 min post-intervention (T2)
  3. Passive hip extension (PHE) angle measured using PHE test performed using a digital inclinometer (or goniometer) to assess hip extension range of motion and muscle flexibility. at baseline (T0), immediately post-intervention (T1), and 15 min post-intervention (T2)
Key secondary outcome measure(s)
Completion date30/03/2023

Eligibility

Participant type(s)
Age groupAdult
Lower age limit18 Years
Upper age limit35 Years
SexAll
Target sample size at registration90
Total final enrolment90
Key inclusion criteria1. Healthy young adults
2. ≥18 years and able to provide written informed consent
3. Physically active (engaging in physical activity at least twice per week) but not competitive athletes
4. Hamstring flexibility ≥60° on passive straight leg raise at baseline
5. Able to perform study procedures
6. Including static stretching protocols and single-leg drop jumps from a 40-cm box in a laboratory setting
Key exclusion criteria1. History of musculoskeletal injury or lower limb surgery within the past 6 months
2. Presence of neurological, cardiovascular, or systemic disorders affecting movement or performance
3. Regular participation in structured flexibility or stretching programs (≥3 times per week)
4. Current pain or discomfort in the lower limbs or spine
5. Use of medications that could influence muscle tone, balance, or coordination
6. Inability or unwillingness to comply with study procedures or provide informed consent
Date of first enrolment01/09/2022
Date of final enrolment30/03/2023

Locations

Countries of recruitment

  • Italy

Study participating centres

Results and Publications

Individual participant data (IPD) Intention to shareNo

Editorial Notes

19/11/2025: Trial's existence confirmed by University of Palermo Bioethical Committee.