Orienteering camp training and youth wellbeing: tracking stress, fatigue and emotions over 4 weeks

ISRCTN ISRCTN14846325
DOI https://doi.org/10.1186/ISRCTN14846325
Secondary identifying numbers YSXTZK2022019
Submission date
22/08/2025
Registration date
26/08/2025
Last edited
26/08/2025
Recruitment status
No longer recruiting
Overall study status
Completed
Condition category
Other
Prospectively registered
Protocol
Statistical analysis plan
Results
Individual participant data
Record updated in last year

Plain English summary of protocol

Background and study aims
This study examines how a 4-week national orienteering training camp affects teenagers’ bodies and minds compared with usual club training. The researchers will track changes in heart-rate variability (a marker of autonomic recovery), morning salivary cortisol (a stress hormone), mood and sleep across 28 days to see whether the camp supports healthier adaptation.

Who can participate?
Healthy adolescents aged 15–18 years with at least 2 years of official orienteering competition experience and medical clearance for training. If under 18, a parent or legal guardian must give consent and the participant provides assent.

What does the study involve?
Participants either attend the national preparation camp or continue their usual club training. Everyone completes brief assessments on Day 1, Day 14 and Day 28: a 5-minute seated morning heart-rate recording while rested and fasted, a small first-waking saliva sample for cortisol, and short questionnaires on perceived stress and mood. Sleep quality and emotional skills are assessed at the start and Day 28. A simple weekly rating of training effort is also recorded. Procedures are non-invasive and each assessment takes about 20–30 minutes. Usual training continues unchanged.

What are the possible benefits and risks of participating?
Direct benefits are not guaranteed, but regular check-ins may help athletes understand training and recovery. Risks are minimal and relate mainly to normal sports participation, brief discomfort from wearing a chest strap, saliva collection, and time spent on questionnaires. Participation will not change medical care.

Where is the study run from?
National Youth Orienteering Preparation Camp (Jiangsu Province), with academic oversight from the School of Physical Education, Shanxi University (Taiyuan, China)

When is the study starting and how long is it expected to run for?
May 2024 to August 2024

Who is funding the study?
Key Provincial Think Tank for Ideological Research and Database Construction in Shanxi (Commissioned Project YSXTZK2022019)

Who is the main contact?
Dr Haiyan Li, aepigenetic@gmail.com

Contact information

Dr Haiyan Li
Public, Scientific, Principal Investigator

School of Physical Education
Shanxi University
No. 92 Wucheng Road
Taiyuan
030006
China

Phone +86 (0)351 7010255
Email aepigenetic@gmail.com

Study information

Study designProspective longitudinal non-randomized parallel-group interventional study
Primary study designInterventional
Secondary study designNon randomised study
Study setting(s)Fitness/sport facility, Training facility/simulation, University/medical school/dental school
Study typeQuality of life, Efficacy
Participant information sheet 47883_PIS.pdf
Scientific titleAdolescent orienteers aged 15–18 in a 4-week national preparation camp versus matched club-trained peers effects on autonomic function, endocrine stress (salivary cortisol), and emotional state over 28 days
Study acronymY-ORIENT
Study objectivesThe objective is to quantify temporal changes in psychological stress, physical fatigue and emotional states during a 4-week national youth orienteering camp, and compare trajectories with matched club-trained controls. The primary hypothesis is that camp participants will show a greater increase in resting RMSSD from Day 1 to Day 28. Secondary hypotheses are that, over the same period, camp participants will show larger gains in SDNN and positive affect (PANAS-C), greater reductions in salivary cortisol and perceived stress (PSS-10), better sleep (PSQI), and higher emotional intelligence (EISA-24).
Ethics approval(s)

Approved 15/06/2024, Shanxi University Ethics Research Committee (Institutional Review Board) (School of Physical Education, Shanxi University, No. 92 Wucheng Road, Taiyuan, 030006, China; +86 (0)351-7010255; xiaoban@sxu.edu.cn), ref: AD2024-197

Health condition(s) or problem(s) studiedPsychophysiological stress, physical fatigue, and emotional wellbeing in adolescent orienteers during an intensive training period
InterventionA 4-week national orienteering camp curriculum (navigation drills, terrain running, interval sessions, and routine morning assessments) is compared with matched club training as usual. Allocation is by natural group (camp vs club), open label, with assessments on Day 1, Day 14, and Day 28.
Intervention typeBehavioural
Primary outcome measureAutonomic recovery (resting heart-rate variability root mean square of the successive differences [HRV, RMSSD] in ms) measured using a 5-minute seated morning HRV recording under fasted, rested conditions with a Polar H10 chest-strap sensor (Polar Electro Oy, Finland), analysed in Kubios HRV v3.x (Kubios Oy, Finland), at Day 1, Day 14, Day 28; primary endpoint is the change Day 1 to Day 28. (If a 1-lead ECG ≥500 Hz is used instead of the chest strap, the device make/country will be recorded in the site file; analysis remains in Kubios HRV v3.x.)
Secondary outcome measures1. Cardiac autonomic variability (standard deviation of normal-to-normal [SDNN] in ms) measured using the same 5-minute morning HRV protocol with Polar H10 (Polar Electro Oy, Finland) and Kubios HRV v3.x (Kubios Oy, Finland) at Day 1, Day 14, Day 28; change Day 1 to Day 28.
2. Sympathovagal balance (low-frequency/high-frequency [LF/HF] ratio) derived from the same HRV session analysed in Kubios HRV v3.x (Kubios Oy, Finland) at Day 1, Day 14, Day 28; change Day 1 to Day 28.
3. Endocrine stress (morning salivary cortisol, nmol/L) measured by ELISA using the Salimetrics® Salivary Cortisol Kit (Salimetrics LLC, USA; e.g., cat. 1-3002) with absorbance read at 450 nm on a BioTek Epoch 2 microplate reader (Agilent BioTek, USA), from first-waking saliva at Day 1, Day 14, Day 28; change Day 1 to Day 28 (exploratory AUC across 28 days).
4. Perceived stress measured using the Perceived Stress Scale-10 (PSS-10) total score at Day 1, Day 14, Day 28; change Day 1 to Day 28.
5. Affect measured using the Positive and Negative Affect Schedule for Children (PANAS-C) (positive and negative subscales) at Day 1, Day 14, Day 28; change Day 1 to Day 28.
6. Sleep quality measured using the Pittsburgh Sleep Quality Index (PSQI) total score at Day 1 and Day 28; change Day 1 to Day 28.
7. Perceived training load measured using session rating of perceived exertion (Borg CR-10 scale) from weekly training logs over Weeks 1–4; endpoints are weekly means.
8. Trait emotional intelligence measured using the Emotional Intelligence Scale for Adolescents (EISA-24) total score at Day 1 and Day 28; change Day 1 to Day 28.
Overall study start date01/05/2024
Completion date28/08/2024

Eligibility

Participant type(s)Healthy volunteer, Learner/student
Age groupChild
Lower age limit15 Years
Upper age limit18 Years
SexBoth
Target number of participants100
Total final enrolment72
Key inclusion criteria1. Age 15–18 years
2. Active orienteering athlete with ≥2 years of official competition experience, confirmed by records/coach
3. Medically cleared for training, with no contraindications to moderate–vigorous exercise
4. Able and willing to provide morning saliva samples and complete HRV and questionnaires at Day 1, Day 14, and Day 28
5. Written informed consent (and parent/guardian consent plus participant assent if <18 years)
Key exclusion criteria1. Acute illness or injury precluding training or scheduled assessments
2. Medications or conditions affecting autonomic or endocrine measures (e.g., beta-blockers, systemic corticosteroids, untreated thyroid disease)
3. Current febrile illness within 7 days of an assessment timepoint
4. Inability/unwillingness to provide saliva samples or complete HRV and questionnaires per schedule
5. No medical clearance, or lack of consent/assent
6. Pregnancy (for female participants), due to hormonal confounding
Date of first enrolment01/07/2024
Date of final enrolment31/07/2024

Locations

Countries of recruitment

  • China

Study participating centre

National Youth Orienteering Preparation Camp (Hailan Pegasus Watertown)
No. 888 Nanhuan Road
Xinqiao Town
Jiangyin
214426
China

Sponsor information

Shanxi University
University/education

School of Physical Education
No. 92 Wucheng Road
Taiyuan
030006
China

Phone +86 (0)351 7010161
Email xiaoban@sxu.edu.cn
Website http://www.sxu.edu.cn
ROR logo "ROR" https://ror.org/03y3e3s17

Funders

Funder type

Research organisation

Key Provincial Think Tank for Ideological Research and Database Construction in Shanxi (Commissioned Project YSXTZK2022019)

No information available

Results and Publications

Intention to publish date28/08/2025
Individual participant data (IPD) Intention to shareYes
IPD sharing plan summaryAvailable on request
Publication and dissemination planResults will be submitted to a peer-reviewed sports science journal and presented at national/international meetings in youth sport and exercise science. The final manuscript, analysis code, and de-identified data will be shared in a public repository at publication.
IPD sharing planThe datasets generated and/or analysed during the current study will be available upon request from Haiyan Li (aepigenetic@gmail.com). De-identified IPD will include resting HRV metrics (RMSSD, SDNN, LF/HF), morning salivary cortisol, perceived stress (PSS-10), mood (PANAS-C), sleep quality (PSQI), emotional intelligence (EISA-24), weekly session-RPE, and basic demographics/training characteristics, with a site code. Data will become available 6 months after primary results are published and remain available for 3 years. Access will be granted for non-commercial, methodologically sound proposals that include a brief analysis plan, evidence of local ethics approval, and a signed data-use agreement; transfer will occur via secure institutional mechanisms. Anonymisation will remove direct identifiers, offset dates, bin ages within the 15–18 range, and suppress rare combinations to minimise re-identification risk. Because participants include minors, sharing is limited to cases where consent/assent and guardian consent cover de-identified data reuse; where consent does not allow this, only aggregate outputs will be provided and raw IPD will not be shared.

Study outputs

Output type Details Date created Date added Peer reviewed? Patient-facing?
Participant information sheet 26/08/2025 No Yes
Protocol file 26/08/2025 No No
Statistical Analysis Plan 26/08/2025 No No

Additional files

47883_SAP.pdf
47883_PIS.pdf
47883_PROTOCOL.pdf

Editorial Notes

22/08/2025: Study's existence confirmed by Shanxi University Ethics Research Committee (Institutional Review Board).