Efficacy of the antimalarial drugs recommended by the National Malaria Control Programme in Madagascar

ISRCTN	ISRCTN36517335
DOI	https://doi.org/10.1186/ISRCTN36517335
Secondary identifying numbers	MDG-304-G05-M

Submission date: 11/05/2009
Registration date: 29/05/2009
Last edited: 10/01/2020

Recruitment status: No longer recruiting
Overall study status: Completed
Condition category: Infections and Infestations

Prospectively registered

Protocol

Statistical analysis plan

Results

Individual participant data

Plain English summary of protocol

Not provided at time of registration

Contact information

Dr Didier Menard
Scientific

Institut Pasteur de Madagascar
BP 1274
Antananarivo
101
Madagascar

Phone	+261 (0)20 22 412 72
Email	dmenard@pasteur.fr

Study information

Study design	Randomised open-label active-controlled parallel-assignment efficacy trial
Primary study design	Interventional
Secondary study design	Randomised controlled trial
Study setting(s)	Other
Study type	Treatment
Scientific title	Assessment of the efficacy of antimalarial drugs recommended by the National Malaria Control Programme in Madagascar in Plasmodium falciparum and Plasmodium vivax malaria: a randomised open-label active-controlled parallel-assignment efficacy trial
Study objectives	Despite major efforts made by national and international health organisations, malaria remains the most widespread infectious parasitic diseases and one of the most serious global health problems in the world. In sub-Saharan African, the main problem is Plasmodium falciparum drug-resistance, especially with the spread of the parasite resistance to the inexpensive and widely used drugs, such as chloroquine (CQ) or sulphadoxinepyrimethamine (SP), and the major consequence is the use of ineffective antimalarial drugs leading to the increasing malarial incidence and mortality. Therefore, substantial efforts have been made to encourage the monitoring and the evaluation of the antimalarial drugs resistance in the endemic countries for assessing regularly their antimalarial drug policies and ensuring a continued coverage of effective antimalarial treatment. In Madagascar, since 2005, antimalarial treatment is in transition with the elaboration and the implementation of the new national policy for the fight against malaria. The main modifications in term of drugs use has been the withdrawal of CQ in favour of artemisinin combination therapies (ACTs), as first-line (artesunate plus amodiaquine combination, ASAQ) and second-line treatment (artemether plus lumefantrine combination), and the use of the SP for intermittent preventive treatment for pregnant women (IPTp). This choice was guided by the recommendations of World Health Organization (WHO) and unpublished data from a clinical trial conducted on the island of Sainte Marie in 2004, which show 36.9% of treatment failure over the 14-day follow-up period, unadjusted by genotyping. However, with regards to the home treatment of presumed malaria in children (HMM), it has been decided to continue to use pre-packaged chloroquine, either PaluStop® sold by the NGO "Population Service International" (PSI) or Ody Tazomoka® freely distributed at primary public health facilities by Malagasy Ministry of Health (Mal-MoH), as transitory measure until ACTs were available at community level. At present, the implementation of the ACTs as first-line treatment at health centres level is complete on the east coast of Madagascar, with the result that only 24% (31/131) of the health districts in Madagascar are using ACT. Since 1999, the antimalarial drug resistance surveillance system in Madagascar is supported by a national network for the surveillance of malaria resistance (named RER - Réseau d'Etude de la Résistance). The RER was formed as a collaborative effort between the MalMoH and the Malaria Research Unit of the Institut Pasteur de Madagascar (IPM). The strategy of monitoring was initially based on the use of the in vitro assessment of P. falciparum sensitivity to antimalarial drugs and the evaluation of the frequency of genetic markers associated with P. falciparum drug resistance. In vivo studies were also carried out, but these studies were limited geographically, the sample sizes were small and used only a 14-day follow-up period, a series of constraints which may have significantly underestimated the true risk of treatment failure. No data concerning the susceptibility of P. vivax to SP is available. In order to improve the monitoring of antimalarial drug resistance in Madagascar, a new RER was set up in 2006, with the support of the Global Fund. According to the WHO recommendation, this network was based on sentinel sites located within public or private health facilities and selected to be representative of the range of ecological and epidemiologic conditions. This multi-site randomised clinical trial aims to assess the therapeutic efficacy of antimalarial therapies recommended by the National Malaria Control Programme.
Ethics approval(s)	National Ethics Committee of the Ministry of Health of Madagascar approved in February 2006 (ref: 007/SANPF/2007)
Health condition(s) or problem(s) studied	Malaria (P. falciparum and P. vivax)
Intervention	P. falciparum clinical trial: All patients aged between 6 months and 15 years were eligible to be enrolled and were screened for malaria at the primary health centres in the sentinel sites on the basis of history of febrile illness. Once written informed consent was given, patients were enrolled in the study and assigned consecutive patient numbers. Randomisation to treatment group was performed in blocks of three or four, and treatment regimens were allocated by an independent individual, not involved in the analysis of the study. Patients were administered either CQ (10 mg/kg on days 0 and 1, and 5 mg/kg on day 2), AQ (10 mg/kg on days 0, 1, and 2), SP (25 mg/kg sulphadoxine and 1.25 mg/kg pyrimethamine as a single dose on day 0) or ASAQ (AS: 4 mg/kg on days 0, 1, and 2 and AQ:10 mg/kg on days 0, 1, and 2). Patients were directly observed for 30 minutes after treatment, and the dose was readministered if vomiting occurred. Patients who repeatedly vomited their first dose of study medication were excluded from the study. Patients were assessed on days 1, 2, 3, 7, 14, 21 and 28, and any intervening day they were unwell for malaria infection. Blood was obtained by finger prick on all follow-up days and on any unscheduled day to use for analysis of thick and thin blood smears and for storage on filter paper. Thick and thin blood slides were examined by light microscopy for parasites on any day during the 28-day follow-up. Blood slides were read by a microscopist blind to treatment allocation. All slides were controlled by a second microscopist also blind to treatment group and previous diagnosis. Discordant slides were read, blind to treatment group and previous diagnosis, by a third microscopist. Haemoglobin was measured on Day 0 and Day 28 using a HemoCue® haemoglobinometre (HemoCue AB, Sweden). P. vivax clinical trial: All patients included were weighed and their medical histories (including previous antimalarial medication) recorded. Clinical examination, including axillary temperature recording and Giemsa staining of serial thick and thin blood films, was carried out on days 0, 1, 2, 3, 7, 14, 21, and 28. Thick and thin films were read by a skilled microscopist. Patients were treated with the standard CQ regimen (25 mg/kg of body weight per day for 3 days) or the standart SP regimen (25 mg/kg sulphadoxine and 1.25 mg/kg pyrimethamine as a single dose on day 0) and monitored for 28 days. Patients were directly observed for 30 min after treatment, and the dose was readministered if vomiting occurred. Patients who repeatedly vomited their first dose of study medication were excluded from the study. Blood was blotted onto filter paper during follow-up and was stored at 4°C for DNA analysis. The haemoglobin concentration was determined on days 0 and 28 (HemoCue AB, Sweden).
Intervention type	Other
Primary outcome measure	P. falciparum: Treatment outcomes were assessed according to WHO 2003 guidelines as Early Treatment Failure (ETF; danger signs or complicated malaria or failure to adequately respond to therapy on days 0 - 3), Late Clinical Failure (LCF; danger signs or complicated malaria or fever and parasitaemia on days 4 - 28 without previously meeting criteria for ETF), Late Parasitological Failure (LPF; asymptomatic parasitaemia on days 4 - 28 without previously meeting criteria for ETF or LCF), and Adequate Clinical and Parasitological Response (ACPR; absence of parasitaemia on day 28 without previously meeting criteria for ETF, LCF, or LPF). Overall Treatment failure (OFT) was considered as the sum of the ETP, LCT and LPF. Patients classified as having suffered treatment failure were treated with quinine (10 mg/kg three times daily for seven days); however, their response to repeat therapy was not assessed. P. vivax: Treatment outcomes were assessed according to WHO 2001 guidelines as "treatment failure" (TF; clinical deterioration due to P. vivax illness requiring hospitalisation, with parasitaemia and an axillary temperature of greater than or equal to 37.5°C any time between days 3 and 28, or parasitaemia on any day between days 7 and 28, regardless of clinical condition) or "adequate clinical and parasitological response" (ACPR; absence of parasitaemia on day 28 without the criteria for TF having been met previously). Patients with TF were treated with artesunate (4 mg/kg on days 0, 1, and 2) plus amodiaquine (10 mg/kg on days 0, 1, and 2), but their response to rescue therapy was not assessed.
Secondary outcome measures	No secondary outcome measures
Overall study start date	01/02/2006
Completion date	31/07/2007

Eligibility

Participant type(s)	Patient
Age group	Child
Lower age limit	6 Months
Upper age limit	15 Years
Sex	Both
Target number of participants	P. falciparum trial: 1,434; P. vivax trial: 120
Key inclusion criteria	P. falciparum trial: 1. Both males and females, aged between six months and 15 years 2. Monoinfection with P. falciparum at a parasitaemia between 1,000 and 200,000/µl 3. Axillary temperature greater than or equal to 37.5°C 4. Body weight greater than 5 kg 5. Absence of severe malnutrition (defined as a child whose weight-for-height is below 3 standard deviations of less than 70% of the median of World Health Organization [WHO] reference values, or who has symmetrical oedema involving at least the feet) 6. Absence of febrile conditions caused by diseases other than malaria 7. Absence of 'danger signs' (inability to stand, breastfeeding or drink; recent convulsions; lethargy or persistent vomiting) and of severe and complicated malaria 8. Haemoglobin (Hb) greater than or equal to 5 g/dl 9. Informed written consent of parents/guardians 10. Ability to attend stipulated follow-up visits 11. Absence of history of hypersensitivity reactions to any of the drugs being evaluated P. vivax trial: 1. Both males and females, aged greater than 6 months 2. Monoinfection with P. vvax at a parasitaemia above 250/µl 3. History of fever during 48 hours prior to time of recruitment 4. Ability and willingness to participate based on information given to parent or guardian and access to health facility 5. Informed consent
Key exclusion criteria	P. falciparum trial: 1. Aged less than 6 or greater than 16 years 2. Severe malnutrition (defined as a child whose weight-for-height is below 3 standard deviations of less than 70% of the median of WHO reference values, or who has symmetrical oedema involving at least the feet) 3. No slide confirmed infection with P. falciparum or a mixed infection that includes a non P. falciparum species 4. Initial parasite density less than 1,000 or greater than 200,000 asexual parasites per microlitre 5. Presence of general danger signs among children less than 5 years (inability to stand, breastfeeding or drink; recent convulsions; lethargy or persistent vomiting) and of severe and complicated malaria 6. Measured axillary temperature less than 37.5 °C 7. Inability to attend stipulated follow-up visits 8. Unwilling to provide informed consent provided by parent/guardian 9. History of hypersensitivity reactions to any of the drugs being evaluated P. vivax trial 1. Presence of clinical condition requiring hospitalisation 2. Presence of severe malnutrition 3. Pregnancy 4. Significant concomitant febrile illness which would interfere with follow-up 5. Chronic infectious diseases other than malaria (e.g., tuberculosis) 6. Known allergy and/or intolerance to drug(s) being tested
Date of first enrolment	01/02/2006
Date of final enrolment	31/07/2007

Locations

Countries of recruitment

Madagascar

Study participating centre

Institut Pasteur de Madagascar

Antananarivo
101
Madagascar

Sponsor information

Ministry of Health (Madagascar)
Government

c/o Dr Philémon Tafangy
National Malaria Control Programme
Antananarivo
101
Madagascar

Phone	+261 (0)22 233 24
Email	rsecresan@simicro.mg
Website	http://www.sante.gov.mg
"ROR"	https://ror.org/05d0mtf30

Funders

Funder type

Other

The Global Fund to Fight AIDS, Tuberculosis and Malaria (Switzerland) - Round three grant (ref: MDG-304-G05-M)

No information available

Results and Publications

Intention to publish date
Individual participant data (IPD) Intention to share	No
IPD sharing plan summary	Not provided at time of registration
Publication and dissemination plan	Not provided at time of registration
IPD sharing plan

Study outputs

Output type	Details	Date created	Peer reviewed?	Patient-facing?
Results article	therapeutic response results	26/02/2008	Yes	No
Results article	baseline data results	04/04/2008	Yes	No
Results article	clinical efficacy results	01/12/2008	Yes	No

Editorial Notes

10/01/2020: Internal review.