Oral hydroxycitric acid (HCA) supplementation enhances glycogen synthesis in exercised human skeletal 1 muscle
| ISRCTN | ISRCTN45782014 |
|---|---|
| DOI | https://doi.org/10.1186/ISRCTN45782014 |
| Protocol serial number | IRB951222 |
| Sponsor | National Science Council (Taiwan) |
| Funder | National Science Council (Taiwan) (ref: NSC 97-2140-H-166-007-MY2) |
- Submission date
- 20/12/2010
- Registration date
- 21/01/2011
- Last edited
- 21/01/2011
- Recruitment status
- No longer recruiting
- Overall study status
- Completed
- Condition category
- Nutritional, Metabolic, Endocrine
Prospectively registered
Protocol
Statistical analysis plan
Results
Individual participant data
Record updated in last year
Plain English summary of protocol
Not provided at time of registration
Contact information
Dr I-Shiung Cheng
Scientific
Scientific
140, Ming Sheng Road
Taichung City
403
Taiwan
Study information
| Primary study design | Interventional |
|---|---|
| Study design | Randomised placebo controlled crossover trial |
| Secondary study design | Randomised controlled trial |
| Study type | Participant information sheet |
| Scientific title | Oral hydroxycitric acid (HCA) supplementation enhances glycogen synthesis in exercised human skeletal 1 muscle: a randomised placebo-controlled cross-over trial |
| Study acronym | HCA, GLUT4,FAT/CD36 |
| Study objectives | In this study, we tested the hypothesis that oral hydroxycitric acid (HCA) supplementation might increase muscle glycogen stores and increase insulin sensitivity after exercise in humans, based on the evidence from mice studies. |
| Ethics approval(s) | National Taiwan Sport University Ethics Committee approved on the 12th December 2006 |
| Health condition(s) or problem(s) studied | Glycogen synthesis in human skeletal muscle |
| Intervention | Since we aimed to determine the HCA effect on recovery, HCA and placebo meals were provided to the subjects immediately after exercise. HCA trial provided a meal containing 70% carbohydrate content with 500 mg HCA in half litre of drinking water for a 3-hour recovery, whereas the placebo trial (placebo, eight comparisons) provided a diet containing 70% carbohydrate content plus same volume of drinking water. In particular, carbohydrate meal contained 2 g carbohydrate per kilogram of body weight. The decision for HCA dosage was based on previous human report which demonstrated positive ergogenic effect on endurance performance. Subjects performed a 60-mins cycling at 75% VO2max. Muscle biopsy samples, blood samples and gaseous samples were obtained immediately after exercise and during 3 hours after exercise. |
| Intervention type | Drug |
| Phase | Not Applicable |
| Drug / device / biological / vaccine name(s) | Hydroxycitric acid |
| Primary outcome measure(s) |
Glycogen assay. Approximately 25 mg of skeletal muscle from the deep portion of the vastus lateralis was dissolved in 1 N KOH at 75°C for 30 minutes. Dissolved homogenate was neutralised by glacial acetic acid and incubated overnight in acetate buffer (0.3 M sodium acetate, pH to 4.8) containing amyloglucosidase (Boehringer Mannheim, Indianapolis, IN). The reaction mixture was neutralised with 1 N NaOH. Samples were then analysed by measuring glucosyl units by the Trinder reaction (Sigma, St. Louis, MO). |
| Key secondary outcome measure(s) |
Molecular expressions of GLUT4, FAT/CD36, measured with reverse transcription-polymerase chain reaction (RT-PCR) and western blotting |
| Completion date | 30/04/2007 |
Eligibility
| Participant type(s) | Patient |
|---|---|
| Age group | Adult |
| Sex | Male |
| Target sample size at registration | 8 |
| Key inclusion criteria | 1. Healthy male volunteers 2. Aged 22.0 +/- 0.3 years 3. Height 171.3 +/- 0.7 cm 4. Weight 73.7 +/- 1.1 kg 5. Body mass index (BMI) 25.2 +/- 0.5 kg/m2 6. Ventilatory equivalent for oxygen (VEO2) 108 7. Peak 45.7 +/- 1.5 ml/kg/min |
| Key exclusion criteria | Male volunteer's VO2max less than 30 ml/kg/min |
| Date of first enrolment | 01/08/2006 |
| Date of final enrolment | 30/04/2007 |
Locations
Countries of recruitment
- Taiwan
Study participating centre
140, Ming Sheng Road
Taichung City
403
Taiwan
403
Taiwan
Results and Publications
| Individual participant data (IPD) Intention to share | No |
|---|---|
| IPD sharing plan summary | Not provided at time of registration |
| IPD sharing plan |
Study outputs
| Output type | Details | Date created | Date added | Peer reviewed? | Patient-facing? |
|---|---|---|---|---|---|
| Participant information sheet | Participant information sheet | 11/11/2025 | 11/11/2025 | No | Yes |
