ISRCTN ISRCTN58774519
DOI https://doi.org/10.1186/ISRCTN58774519
Secondary identifying numbers 16/LO/1512
Submission date
15/08/2016
Registration date
22/09/2016
Last edited
28/09/2022
Recruitment status
No longer recruiting
Overall study status
Completed
Condition category
Cancer
Prospectively registered
Protocol
Statistical analysis plan
Results
Individual participant data

Plain English Summary

Background and study aims
Pancreatic cancer causes the uncontrolled growth of cells in a large gland of the digestive system. Pancreatic cancer has a very poor prognosis (forcast) with an overall survival rate of less than 5%. Current treatments are ineffective and even if the patient responds to initial treatments, relapse (the cancer coming back) is common due to the survival of small populations of resistant cancer cells. The immune system is capable of recognising and eliminating invading organisms by virtue of differences in their appearance when compared to normal components of the body. Cancer cells also have a different appearance compared to normal cells. However, these differences are often too small and weak to stimulate the immune system sufficiently to respond effectively to eliminate the tumour. The aim of this study is to analyse the small differences between healthy and cancer cells in pancreatic cancer patients by analysing genetic information from pancreatic cancer cells and see their ability to stimulate an immune response.

Who can participate?
Adults aged 17 to 66 who fulfill the NHS blood donation requirements.

What does the study involve?
Blood samples are taken from participants and are analysed for genetic information with respect to their ability to stimulate an immune response against cancer.

What are the possible benefits and risks of participating?
Not provided at time of registration.

Where is the study run from?
1. NHS Blood and Transplant. Blood Donation Centre Edgware (UK)
2. NHS Blood and Transplant. Tooting Blood Donor Centre (UK)

When is the study starting and how long is it expected to run for?
August 2016 to July 2018

Who is funding the study?
Pancreatic Cancer Research Fund (UK)

Who is the main contact?
Professor Yaohe Wang

Contact information

Prof Yaohe Wang
Public

Charterhouse Square
London
EC1M 6BQ
United Kingdom

Study information

Study designObservational
Primary study designObservational
Secondary study designCross sectional study
Study setting(s)Other
Study typeScreening
Participant information sheet Not available
Scientific titleIdentification of immunogenic neo-epitopes for the development of personalised pancreatic cancer vaccines
Study hypothesisSequence analysis has allowed us to develop a peptide library of neo-epitopes that are expressed at high frequency in patient populations and have high binding affinities compared to their wild-type counterpart to HLA-A2, HLA-DP4, HLA-E*01:01 or HLA-*01:03 molecules. We hypothesise that a number of these will be sufficiently immunogenic to stimulate a T cell interferon-γ (IFN-γ) response in vitro, that will translate to an in vivo anti-tumour response. Immunogenic neo-epitopes can then be combined in a peptide vaccination program using adjuvants such as oncolytic viruses for targeted delivery and expression within tumours of PDAC patients to stimulate robust and long-term anti-tumour responses.
Ethics approval(s)The Proportionate Review Sub-committee of the London - Westminster Research Ethics Committee, 10/08/2016, ref: 16/LO/1512
ConditionPancreatic cancer
InterventionPeripheral blood mononuclear cells (PBMCs) obtained from leukocyte filters from healthy individuals will be HLA typed using commercially available reagents from thermofisher scientific. HLA-A2, HLA-DP4, HLA-E*01:01 and/or HLA-E*01:03 positive samples will be pulsed with peptides selected after bioinformatics analysis of available sequence data. IFN-γ and interleukin-2 (IL-2) production by the T cells in the samples will be evaluated by ELISA after two rounds of stimulation within a two weeks time as a measure of peptide immunogenicity. Once immunogenic peptides have been identified, their wild-type counterparts will be analysed in parallel to confirm specificity for the mutated epitope. Immunogenic peptides whose wild-type counterparts do not elicit immune responses will then be selected for inclusion in an oncolytic virus-based vaccine to be analysed in vivo using transgenic HLA-A2/HLA-DP4 mice.
Intervention typeOther
Primary outcome measureImmunogenicity of neo-epitope candidates selected from available mutanome data using peripheral blood mononuclear cells (PBMCs) from healthy individuals.
Secondary outcome measuresN/A
Overall study start date01/08/2016
Overall study end date20/07/2018

Eligibility

Participant type(s)Healthy volunteer
Age groupAdult
SexBoth
Target number of participantsAbout 100 individuals.
Participant inclusion criteriaIndividuals who fulfill the NHS BT requirements for blood donation, which are:
1. fit and healthy
2. weigh over 7 stone 12 lbs or 50kg
3. are aged between 17 and 66 (or 70 if you have given blood before)
4. are over 70 and have given blood in the last two years
Participant exclusion criteriaSame as NHS BT criteria, which are:
1. receiving treatment
2. taking medication
3. travelling outside of the UK
4. tattoos
5. pregnancy
6. illness
7. cancer
8. received blood, blood products or organs
Recruitment start date01/08/2016
Recruitment end date20/07/2018

Locations

Countries of recruitment

  • England
  • United Kingdom

Study participating centres

NHS Blood and Transplant. Blood Donation Centre Edgware
Edgware Community Hospital
Burnt Oak Broadway
Edgware
Middlesex
HA8 0AD
United Kingdom
NHS Blood and Transplant. Tooting Blood Donor Centre
75 Cranmer Street
Tooting
London
SW17 0RB
United Kingdom

Sponsor information

Joint Research Management Office, Queen Mary University of London
University/education

5 Walden Street - Lower Ground Floor
London
E1 2EF
England
United Kingdom

ROR logo "ROR" https://ror.org/026zzn846

Funders

Funder type

Charity

Pancreatic Cancer Research Fund
Private sector organisation / Trusts, charities, foundations (both public and private)
Alternative name(s)
PCRF
Location
United Kingdom

Results and Publications

Intention to publish date20/07/2019
Individual participant data (IPD) Intention to shareYes
IPD sharing plan summaryOther
Publication and dissemination planThe results of this study will be published in suitable peer-reviewed scientific journals.
IPD sharing plan-

Study outputs

Output type Details Date created Date added Peer reviewed? Patient-facing?
Results article 27/05/2022 28/09/2022 Yes No
HRA research summary 28/06/2023 No No

Editorial Notes

28/09/2022: Publication reference added.
06/06/2017: Plain English summary added.
10/04/2017: Internal review.